Table 3 The median relative standard deviation (RSD) of the individual compounds in HOS cells and the percentage of compounds with a RSD < 15% threshold (in total and in % from detected number) for liquid chromatography (LC) and flow injection analysis (FIA) mass spectrometry.

From: Analysis of adherent cell culture lysates with low metabolite concentrations using the Biocrates AbsoluteIDQ p400 HR kit

Measure type

Lysis buffer

EtOH-P

EtOH

MeOH

LC

Median RSD [%]

25

9

11

Compounds with RSD < 15% [%]

3/26 (12%)

23/27 (85%)

23/26 (88%)

FIA

Median RSD [%]

43

23

30

Compounds with RSD < 15% [%]

3/24 (13%)

16/77 (21%)

4/55 (7%)

  1. Five replicates were measured in each condition; outliers were removed from the final analysis as described in the materials and methods.
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