Figure 2

The systems approach for YbdO and YbeF transcriptional factors function prediction. (A) The genome-wide binding of YbdO. (B) The zoom-in of the binding site at the promoter of the gene ybdO. (C) Transcriptomic data for citC (citrate transporter) (D) citCDEFXGT (citrate lyase encoding) genes clustering with ybdO-ybdMN, and ybeF, the analysis across the closely related bacteria. dcuC-anaerobic dicarboxylate transporter, lipA- lipoyl synthase (E) The growth measurement of the ybdO mutant compared to the wild type BW25113 strain of E. coli in 96-well plates microaerobically in M9 glucose supplemented by 30 mM citrate at pH 7.5 (orange and grey line) or glycerol as carbon source (blue and yellow line). (F) Growth was measured in 96-well plates (M9 with glycerol) as the carbon source, supplemented by 30 mM citrate at pH 6.5 (WT-orange and ybdO mutant strain-blue line).