Figure 5
The experimental validation of the transcription factor YneJ (PtrR). (A) Overview of two alternative pathways of putrescine utilization in E. coli. PtrR-regulated genes are shown in yellow boxes. Transporters are shown in blue. Abbreviations: GABAld – gamma-aminobutyraldehyde, PatA—putrescine aminotransferase, PatD—gamma-aminobutyraldehyde dehydrogenase, YneI (Sad)—succinate-semialdehyde dehydrogenase, YneH (GlsB)—glutaminase. GadAB- two glutamate decarboxylase isoforms, GabD, Sad (YneI)-succinate-semialdehyde dehydrogenase, GlsB (YneH), glutaminase. (B) The growth measurement of the ptrR, yneH mutants compared to the wild type BW25113 strain of E. coli. The cell cultures were grown in M9 medium with 10 mM Glu/10 mM Ptr, as nitrogen sources and 0.4% glycerol (v/v) as the primary carbon source or (C) M9 glucose medium. (D) Transcriptomic data for sad (yneJ) and glsB (yneH) at different growth conditions for E. coli MG1655 strain and adapted MG1655 derivatives (iModulonDB, PRECISE2). The activation of the sad and glsB promoter at the ptrR mutant strain was detected.
