Figure 4

Specificity of aptamer-loaded beads. Capturing and elution results of APC using MyOne beads coupled to APC-specific HS02-52G aptamer, negative control sequence AD02-52, or non-coupled MyOne beads. The amount of APC which was captured from binding buffer containing 10 µg APC in 500 µl of binding buffer (5 µg/ml) and released during consecutive steps of washing (W1 to W3) or elution (E1 to E3) was quantified by an activity assay using an APC standard curve. Data are shown as mean ± SD of three measurements.