Figure 5

Capturing and elution of APC using HS02-52G coupled MyOne beads. Different amounts of APC were added to 500 µl binding buffer followed by APC capturing with MyOne beads coupled to HS02-52G aptamer and elution using elution buffer containing 5 mM EDTA. The eluted APC in consecutive elution steps (E1 to E3) was quantified by an activity assay using an APC standard curve. Data are shown as mean ± SD of three measurements.