Figure 8 | Scientific Reports

Figure 8

From: Army liposome formulation containing QS-21 render human monocyte-derived macrophages less permissive to HIV-1 infection by upregulating APOBEC3A

Figure 8

Expression of anti-viral genes is upregulated in ALFQ-treated MDM. Primary human monocytes were differentiated into MDM following in vitro culture with M-CSF media. At day 4 post-culture, the cells were treated with ALF55 or ALFQ for 24 h. Untreated MDM cultures served as controls. Cells were harvested, lysed and the gene expression levels of the cellular restriction factors were analyzed by qRT-PCR. The dot plots show fold-change in the expression levels of HIV-1 restriction genes for MDM that were pre-treated with ALF55 (brown circles) or ALFQ (magenta circles) in relation to the untreated (no adjuvant) MDM from triplicates wells of 4 donors. Statistical differences between the two groups were determined by two-tailed Mann–Whitney test.

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