Figure 9 | Scientific Reports

Figure 9

From: Army liposome formulation containing QS-21 render human monocyte-derived macrophages less permissive to HIV-1 infection by upregulating APOBEC3A

Figure 9

Protein synthesis of APOBEC3A and IFI16 is induced in ALFQ-treated MDM. Primary human monocytes were differentiated into MDM following in vitro culture with M-CSF media. At day 4 post-culture, the cells were treated with ALF55 or ALFQ for 24 h. Untreated (no adjuvant) MDM cultures served as controls. Cells were harvested, lysed, and analyzed by SDS-PAGE followed by Western blotting using antibodies against SAMHD1, phospho-SAMHD1, IFI16 and APOBEC3A. (a) Cropped images of the blots shows the presence of IFI16 protein and APOBEC3A protein only in lysates from ALFQ-treated MDM. (b) Densitometry analysis was performed on protein bands (APOBEC3A, IFI16, SAMHD1, and phospho-SAMHD1), and normalized to its corresponding GAPDH band. The original blots are presented in Supplementary Figs. 1–5.

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