Figure 4 | Scientific Reports

Figure 4

From: Pre-transplant infusion of donor leukocytes treated with extracorporeal photochemotherapy induces immune hypo-responsiveness and long-term allograft survival in murine models

Figure 4

Treatment with ECP-DL inhibits the production of donor-specific antibodies (DSA) following heterotopic heart transplants. After heart transplantation (BALB/c to B6), plasma samples were collected from B6 recipient mice and assessed for DSA at POD5, 7, 14, 21, and 28 (n = 3–8 for each timepoint). Transplant recipients either received ECP-DL at day -7 or no treatment. Production of all DSA subtypes was lower in ECP-DL treated animals at all time points compared to control. Minimal DSA production in the ECP-DL group at POD5 suggests that the animals are not sensitized by ECP-DL infusion. MFI: Mean fluorescence intensity; *p < 0.05, **p < 0.01 by two-way ANOVA, followed by classical t-tests.

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