Figure 9
Concordant Kir6.2 knockout plasma and tissue metabolome. (A) Overlap between tissue and corresponding plasma of Kir6.2 knockout (KO; n = 10) versus wildtype (WT; n = 10) metabolomes identified 15 shared concordant differential metabolites. Overlapping core metabolites are members of distinct metabolic pathways, primarily associated with amino acid and lipid metabolism. (B) WT and KO plasma and tissue spectral intensities were cross-referenced for p-cresol sulfate (left) and N-acetylornithine (right), the top ranking metabolites upregulated and downregulated in KO versus WT as modeled by soft independent modeling of class analogy variable importance in projection (VIP) scoring and Random Forest (RF) classification. (C) Plasma and tissue Kir6.2 dependent metabolomes yielded matching disease and disorder profiles, with (D) equivalent magnitude of predicted cardiovascular adverse outcome.
