Figure 5 | Scientific Reports

Figure 5

From: ROCK 1 and 2 affect the spatial architecture of 3D spheroids derived from human corneal stromal fibroblasts in different manners

Figure 5

Effects of ROCK-is on the mRNA expression of TIMPs (1–4) and MMPs (2, 3, 9, 14) in 3D spheroids of human corneal stroma fibroblasts (HCSFs). In the absence or presence of 1 μM or 10 μM ROCK-is, ripasudil (Rip) or KD025, 3D HCSFs spheroids at Day 6 were subjected to qPCR analyses and the mRNA expression of TIMPs (1–4) and MMPs (2, 3, 9, 14) was estimated. All experiments were performed in duplicate using fresh preparations (n = 10–15, each). Data are presented as the arithmetic mean ± standard error of the mean (SEM). *P < 0.05, **P < 0.01, ***P < 0.005, ***P < 0.001 (ANOVA followed by a Tukey’s multiple comparison test).

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