Table 1 Summary of the effects of ROCK 1 and ROCK 2 on the physical properties of 3D human corneal stroma fibroblasts (HCSFs) spheroids and gene expression of 2D and 3D cultured HCSFs.
1 mM Rip | 10 mM Rip | 1 mM KD025 | 10 mM KD025 | |
|---|---|---|---|---|
Size | ↑↑↑↑ | ↑↑↑↑ | (–) | ↑↑ |
Stiffness | (–) | ↓↓ | (–) | ↑ |
COL 1 | ||||
2D | (–) | (–) | (–) | ↓ |
3D | ↓↓↓↓ | ↓↓↓↓ | ↓ | ↓↓↓↓ |
COL 4 | ||||
2D | (–) | ↓ | (–) | ↑↑ |
3D | (–) | ↓ | (–) | (–) |
COL 6 | ||||
2D | (–) | (–) | (–) | (–) |
3D | (–) | (–) | ↑↑↑ | (–) |
FN | ||||
2D | (–) | ↓↓↓↓ | (–) | (–) |
3D | ↓↓ | ↓↓↓↓ | ↓↓ | ↓↓↓↓ |
TIMP1 | ||||
2D | (–) | (–) | (–) | (–) |
3D | ↓↓ | ↓↓↓↓ | ↓↓↓↓ | (–) |
TIMP2 | ||||
2D | (–) | (–) | (–) | ↓↓↓↓ |
3D | ↓↓↓ | ↓↓↓↓ | (–) | (–) |
TIMP3 | ||||
2D | (–) | ↑↑ | (–) | ↓↓ |
3D | (–) | ↓↓↓↓ | (–) | ↓↓↓↓ |
TIMP4 | ||||
2D | ↓↓ | (–) | ↑↑↑↑ | (–) |
3D | (–) | ↑↑↑↑ | (–) | (–) |
MMP2 | ||||
2D | (–) | (–) | (–) | ↓↓↓↓ |
3D | ↓ | (–) | (–) | (–) |
MMP3 | ||||
2D | (–) | (–) | ↑↑↑↑ | ↑↑↑ |
3D | (––) | ↑↑↑↑ | (–) | (–) |
MMP9 | ||||
2D | (–) | (–) | (–) | (–) |
3D | (–) | (–) | ↓↓↓↓ | ↓↓ |
MMP14 | ||||
2D | (–) | (–) | (–) | ↓↓↓↓ |
3D | (–) | ↑↑↑↑ | (–) | (–) |
GRP 78 | ||||
2D | (–) | (–) | (–) | (–) |
3D | (–) | (–) | ↓ | ↓↓↓↓ |
GRP 94 | ||||
2D | (–) | ↓↓ | ↓↓↓ | ↓↓↓↓ |
3D | ↑ | (–) | ↓↓↓↓ | ↓↓↓↓ |
IRE 1 | ||||
2D | (–) | (–) | (–) | ↓ |
3D | (–) | (–) | (–) | (–) |
ATF 6 | ||||
2D | (–) | (–) | (–) | ↓↓ |
3D | (–) | (–) | ↓↓↓ | ↓↓↓↓ |
PERK | ||||
2D | (–) | (–) | (–) | ↓↓ |
3D | (–) | (–) | ↓↓↓↓ | ↓↓↓↓ |
ATF 4 | ||||
2D | (–) | ↓↓↓↓ | ↓↓↓↓ | ↓↓↓↓ |
3D | (–) | (–) | ↓↓↓↓ | ↓↓↓↓ |
XBP 1 | ||||
2D | (–) | (–) | (–) | ↓↓ |
3D | (–) | ↓ | (–) | ↓↓↓↓ |
sXBP 1 | ||||
2D | (–) | (–) | (–) | ↑ |
3D | ↓↓ | ↑↑ | ↓↓↓↓ | ↓↓↓↓ |
CHOP | ||||
2D | (–) | (–) | (–) | ↓↓↓↓ |
3D | (–) | ↓ | ↓↓ | ↓↓↓↓ |
