Figure 2

Colocalization analysis of DBRs for human and viral molecular transducers, MS-associated SNPs and DNA regulatory regions derived from databases. (A) Schematic representation of the colocalization analysis. (ROI region of interest, DBR DNA binding regions, ENCODE TFBS transcription factor binding site). The figure shows the tracks we considered for the colocalization analyses. In brief, the ROI included the DBRs of MS-related viral and human transducers and was matched with MS-associated SNPs extended by 50, 100, and 200 kilobases that colocalize with regions plausibly coding for trRNAs (Database). As a control (Universe), we took from ENCODE the entire list of transcription factors binding sites. Results were considered significant if a colocalization was found across ROI and a Databases element without occurring in the Universe as a statistically significant match. (B–E), Colocalization results of EBNA2, EBNA3C, AID, VDR. The charts display results of all matches, i.e., with MS-associated SNPs and their extension at ± 50, 100, 200 kb. X-axis shows the Odd Ratio, y-axis shows the − log (pValue); dot size is proportional to to the Harmonic Score (HS). Thus, prioritized hits are represented by dots that occupy the upper-right area of the chart. Dots are coloured by cell type. Top-scoring hits in each subgroup are labeled; labels were arbitrarily designated according to the database of origin and the cell lineage where the enrichment occurred.