Figure 2

Effects of medium and embryokine treatments on gestation length and calf birth weight. Embryokine treatments were added at day 5 of culture and were vehicle, colony stimulating factor 2 (CSF2) and dickopf WNT signaling pathway inhibitor 1 (DKK1). Gestation length (A) was affected by embryokine (P = 0.024) and the interaction between medium and embryokine (P = 0.031). Moreover, the contrast comparing vehicle vs DKK1 across media was significant (P = 0.017). Birth weights for individual male calves after adjustment for day of age at weighing and sire are shown in panel (B) while results of statistical analysis are in panel (C). There were no treatment effects on birth weight. Results in panels (A,C) are least-squares means ± SEM.