Table 6 Flame atomic absorption spectrometry for heavy metals analysis of culture filtrate samples during shake-flask batch cultivation and RP leftover samples during shake-flask, and uncontrollable pH batch cultivation system.

From: A sustainable and effective bioprocessing approach for improvement of acid phosphatase production and rock phosphate solubilization by Bacillus haynesii strain ACP1

Metals conc. (mg L−1)

Incubation time (h)

0

4

12

20

28

32

Flame atomic absorption spectrometry for culture filtrate

K

158.29

227.19

365.4

484.99

506.34

351.74

Pb

 < 0.3535

 < 0.353

 < 0.353

1.455

0.353

 < 0.353

Fe

1.890

3.62

10.081

38.26

15.230

12.757

Cd

0.0222

0.0373

0.1276

0.155

0.0437

0.047

Zn

1.49

0.350

0.6989

1.375

0.874

0.2183

Mg

83.1

95.47

123.98

246.45

127.46

85.46

Mn

0.9

1.46

2.157

3.727

4.19

3.55

Ca

349.88

536.36

660.53

709.44

633.28

458.219

Na

611.20

1035.01

1825.59

1135.26

795.18

641.50

Cu

 < 0.0346

 < 0.0346

0.0535

0.1339

0.293

0.0601

Metals conc. (mg Kg−1)

Rock phosphate sample (control)

Shake flask batch cultivation

Uncontrollable pH batch cultivation

   

Flame atomic absorption spectrometry for RP leftover samples

   

Co

10.32

9.616

10.01

   

K

9777.00

2166.00

4186.36

   

Fe

105,944.4

145,140

43,902

   

Ni

24.420

20.420

1.58

   

Cd

0.968

1.1564

0.47

   

Cr

15.29

7.0880

32.3400

   

Zn

1255.5

95.52

7254.54

   

Mg

28,944.400

17,454.00

57,286.300

   

Mn

364.5

1944.0

5754.00

   

Li

77.77

1176.0

3450.00

   

Ca

153,629.6

229,000

135,272.72

   

Sn

10.120

 < 1.2

 < 1.2

   

Ag

288.8

426

1.236

   

Na

16.60

11,430

19,145.45

   

Cu

1422.22

13.90

20.81

   
  1. Significant values are in [bold].