Figure 4
From: Peanut triacylglycerols activate innate immunity both in insects and mammals
The peanut TAGs induce TNF-α production by mammalian macrophage cells. Left panel. The concentration of TNF-α present in the culture media of RAW264 cells were determined as described in “Materials and Methods”. After 6 h of co-incubation, the peanut TAGs induced TNF-α production by RAW264 cells in a dose-dependent manner. The y-axis represents the TNF-α concentration in the culture media, and the x-axis represents the dose of peanut TAGs added to the cell culture. A result of triplicate experiment is shown in the char (each plot represents one replicate). There was a significant difference across samples in the analysis of variance (ANOVA, p < 0.001), and each of the three doses resulted in statistically significant increases as demonstrated by the post-hoc t-tests (p < 0.001). Right panel. The concentration of TNF-α present in the culture media of RAW264 cells, when co-incubated with 0.5 μg/mL Lipopolysaccharide (LPS) for 6 h, were determined. The y-axis represents the TNF-α concentration in the culture media. A result of triplicate experiment is shown in the chart (each plot represents one replicate).
