Figure 5
The SNP on Or and the examination of its effect on carrot root color in breeding line C. (a) The SNP detected on Or between parental plants in F2 populations A and B. The SNP causes an amino acid substitution. The upper sequence is identical to the reference sequence of Iorizzo et al.10, and the lower sequence is a new allele of Or. (b) The allelic effect of the SNP on Or in another breeding line at Fujii Seed. TT and TG showed TT homozygote and heterozygote of the Or SNP, respectively. Carrot root color was visually evaluated as three grades: dark, middle, and light orange color (b,c). All of the plants with dark or middle orange color roots showed a heterozygote for the Or SNP, and all plants with the slightly light orange color roots showed TT homozygote for the Or SNP. (c) Examples of carrot root color visually evaluated as three grades.
