Figure 6
The phylogenetic tree based on the amino acid sequences of LCYE, LCYB, CYC-B, NSY, and CCS, and the amino acid substitutions between the parental plants of F2 population A on DCAR_022896. (a) The phylogenetic tree was drawn by the neighbor-joining method based on the amino acid sequences of Daucus carota DCAR_022896, D. carota LCYE (DCAR_028276), Arabidopsis thaliana LCYE (At5g57030), D. carota LCYB (DCAR_020544), A. thaliana LCYB (At3g10230), Solanum lycopersicum CYC-B, Carica papaya CYC-B (evm.model.supercontig_195.16), Citrus sinensis CYC-B (orange1.1g010693m.g), D. carota NSY (DCAR_017191), D. carota NSY(2) (DCAR_025914), A. thaliana NSY (At1g67080), S. lycopersicum NSY (CAB93342.1), Capsicum annuum CCS (Q42435.1), and Lillium lancifolium CCS (JF304153). Values at the nodes indicate the percentage consensus support as calculated using a bootstrapping test with 1,000 replications. (b) Five amino acid substitutions (black filled circle) were detected between seed (Fs001) and pollen (Fs002) parents of F2 population A on DCAR_022896. Similar substitutions are shown in gray background.
