Figure 1 | Scientific Reports

Figure 1

From: Non-canonical Wnt signaling participates in Jagged1-induced osteo/odontogenic differentiation in human dental pulp stem cells

Figure 1

Indirect immobilized Jagged1 promotes hDPSC osteo/odontogenic differentiation. Mesenchymal stem cell surface markers were examined using (a) flow cytometry. Multilineage differentiation towards the osteo/odontogenic and adipogenic lineages was examined using (b) alizarin red s staining and (c) oil red o staining, respectively. Cells were seeded on Jagged1 immobilized surface and maintained in osteo/odontogenic medium. Cells on hFc-immobilized surfaces were used as the control. The cells were cultured in osteo/odontogenic medium for 3, 7, and 14 days. Osteo/odontogenic differentiation was evaluated by (d) ALP staining and (e) in vitro mineral deposition using alizarin red s staining. The alizarin red s staining was solubilized, and (f) the absorbance was measured at 570 nm. Bars indicate a significant difference between groups (p < 0.05).

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