Figure 2
From: Robust and scalable barcoding for massively parallel long-read sequencing
A barnyard experiment (two species) is used to assess the feasibility and performance of NS-watermark barcoding for massively parallel sequencing. A subset of 3840 NS-watermark barcodes, from a set of 4096, is synthesized in a microarray and cleaved to form an oligo pool, which is further divided into two subpools of 1920 barcodes each, followed by preferential amplification of one strand. The resulting ssDNA-enriched subpools are used to tag B. Pertussis (sample A) and D. mojavensis (sample B) amplicons. Barcoded amplicon subpools A and B are pooled in a molar ratio of 1:2 and sequenced together on the MinION sequencing platform.
