Figure 3
From: Robust and scalable barcoding for massively parallel long-read sequencing
Analysis of raw sequencing output. (a) Alignment of raw reads to the expected barcoded construct for amplicon A, which includes forward and reverse species-specific primers (FPA/RPA), along with a highly-variable barcode region, flanking sequences CSA and BPFA, and auxiliary sequences CGC and A, or the reverse complement (rc) thereof. Only reads aligning in the forward sense are shown to highlight that about half the reads are trimmed ~25 bp shorter at the end. (b) Read-length histogram of raw reads with ground truth assignment to pools A or B. Dotted lines show the window used for length filtering. Reads outside the window or with either unknown or ambiguous ground truth were not considered and are shown in grey. (b-inset) Zoom in on the valid length window, showing secondary peaks (bimodality) due to imperfect adapter trimming.
