Figure 6
From: No-ozone cold plasma can kill oral pathogenic microbes in H2O2-dependent and independent manner
The NCP-mediated increase of H2O2 level in the medium is crucial for the bactericidal effect of NCP in liquid medium. (A) The results of the H2O2 concentration measurement. NCP was treated on 1 ml of liquid medium for 0.5, 1, 3, 5 min, and right after the treatment, the H2O2 level of the medium was detected using Amplex red assay kit. (B) The liquid medium (1 ml) samples were subjected to un-mounted NCP, NCP mounted with capillary or microcapillary tips for 5 min. After the treatment, H2O2 level in the medium was detected. (C) The results of nitrite level detection using Griess assay kit. The liquid medium (1 ml) samples were subjected to the NCP treatment for 1, 3, 5 min, and the NO2− level in the medium was detected right after the treatment. (D) The results of the experiments monitoring the effect of NAC on the NCP-mediated bactericidal effect against S.mutans. The liquid medium samples containing S. mutans were treated with NCP for 5 min in presence or absence of NAC at concentration of 0.2, 0.4, and 1 mM respectively. The treated samples were incubated for 24 h further, and plated on solid medium. At 24 h after the additional incubation, CFU was counted. Data shown are the average of three independent experiments, **p < 0.05, **< 0.01, ***< 0.001.
