Figure 1 | Scientific Reports

Figure 1

From: LincRNA#1 knockout alone does not affect polled phenotype in cattle heterozygous for the celtic POLLED allele

Figure 1

Comparison of uninjected and microinjected zygote development rates and mutation efficiencies. Zygotes were microinjected with gRNA/Cas9 ribonucleoproteins targeting the 5′ and 3′ regions surrounding lincRNA#1 at 6 h post insemination. Blastocyst development rate of uninjected control (green) and microinjected embryos when targeting the (a) 5′ and (b) 3′ regions surrounding lincRNA#1. Percentage of Cas9-induced mutations in blastocysts when injected with ribonucleoproteins targeting the (c) 5′ and (d) 3′ regions surrounding lincRNA#1. Error bars = SEM. **P < 0.01.

Back to article page