Figure 2 | Scientific Reports

Figure 2

From: LincRNA#1 knockout alone does not affect polled phenotype in cattle heterozygous for the celtic POLLED allele

Figure 2

Comparison of uninjected and microinjected zygote development rates and mutation and knockout efficiencies. Zygotes were microinjected with Cas9 protein and either linc 5′g1 and linc 3′g1 (Co1), or linc 5′g2 and linc 3′g1 (Co2), at 6 h post insemination. (a) Percentage of uninjected control (green) and microinjected zygotes that reached the blastocyst stage of development. (b) Mutation rates and (c) knockout rates in embryos injected with gRNA/Cas9 ribonucleoproteins for Co1 (blue) and Co2 (yellow) injection groups. Blastocysts were classified as mutated if a mutation occurred in at least one target site. (d) Type of lincRNA#1 knockout (%) in injected embryos. Bi = biallelic (aqua); Mosaic (purple). Error bars = SEM. *P < 0.05.

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