Figure 5
Histological analysis of a representative fetus harvested from embryo transfer (ET) 2 alongside horned and polled controls. (a–c) Fontal skin and (d–f) horn bud region of age matched horned and polled control fetuses alongside fetus 2, a representative biallelic lincRNA#1 knockout fetus from ET2, at 90 days of gestation. Multiple layers of vacuolated keratinocytes and nerve bundles (black stars) can be seen in the horn bud region of the horned control fetus, and hair follicles can be seen in the frontal skin of all fetuses as well as the horn bud regions of the polled control and the knockout fetus (yellow arrows).
