Table 1 Embryo transfer (ET) results from zygotes injected 6 h post insemination with Cas9 protein and gRNAs linc 5′g1 and linc 3′g1.

From: LincRNA#1 knockout alone does not affect polled phenotype in cattle heterozygous for the celtic POLLED allele

ET

Genotype at the POLLED loci

Blastocysts transferred

Recipients

35 days of gestation

75 days of gestation

Viable fetuses (%)

Fetuses harvested (%)

Pregnant (%)

Fetuses detected (%)

Pregnant (%)

Fetuses detected (%)

1

pp

9

3

2 (67)

5 (56)

2 (67)

5 (56)

0 (0)

5 (56)

PCP

15

5

1 (20)

2 (13)

0 (0)

0 (0)

2

PCP

15

5

4 (80)

5 (33)

4 (80)

5 (33)

5 (33)

5 (33)

Total

pp

9

3

2 (67)

5 (56)

2 (67)

5 (56)

0 (0)

5 (56)

PCP

30

10

5 (50)

7 (23)

4 (40)

5 (17)

5 (17)

5 (17)

  1. Three blastocysts were transferred per recipient. Confirmation of pregnancies was performed on day 35 of gestation and fetal viability at 75 days of gestation. Nonviable fetuses were harvested immediately, and viable fetuses were harvested at 90 days of gestation.