Table 1 Embryo transfer (ET) results from zygotes injected 6 h post insemination with Cas9 protein and gRNAs linc 5′g1 and linc 3′g1.
ET | Genotype at the POLLED loci | Blastocysts transferred | Recipients | 35 days of gestation | 75 days of gestation | Viable fetuses (%) | Fetuses harvested (%) | ||
|---|---|---|---|---|---|---|---|---|---|
Pregnant (%) | Fetuses detected (%) | Pregnant (%) | Fetuses detected (%) | ||||||
1 | pp | 9 | 3 | 2 (67) | 5 (56) | 2 (67) | 5 (56) | 0 (0) | 5 (56) |
PCP | 15 | 5 | 1 (20) | 2 (13) | 0 (0) | 0 (0) | – | – | |
2 | PCP | 15 | 5 | 4 (80) | 5 (33) | 4 (80) | 5 (33) | 5 (33) | 5 (33) |
Total | pp | 9 | 3 | 2 (67) | 5 (56) | 2 (67) | 5 (56) | 0 (0) | 5 (56) |
PCP | 30 | 10 | 5 (50) | 7 (23) | 4 (40) | 5 (17) | 5 (17) | 5 (17) | |