Figure 3 | Scientific Reports

Figure 3

From: Identification of an interactome network between lncRNAs and miRNAs in thyroid cancer reveals SPTY2D1-AS1 as a new tumor suppressor

Figure 3

SPTY2D1-AS1 overexpression suppresses cell viability, migration, and invasion in vitro. Cal62 cells were transfected with an SPTY2D1-AS1 (SPTY) expression vector or the empty vector (A) SPTY levels relative to the empty vector-transfected cells after 72 h of transfection. (B) XTT cell viability assay at the indicated times points. (C) Upper panel: representative images of crystal violet-stained colonies. Bottom panel: quantification of crystal violet absorbance. (D) Migration assay. Left panel: representative images of a wound-healing assay. Right panel: quantification at the indicated time points after scratching. (E) Cell invasion assay. Upper panel: representative images of the lower chamber (invading cells). Bottom panel: cell invasion quantification relative to empty vector-transfected cells. Values represent mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.

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