Table 2 Similar to Table 1, this table summarizes previous screenings of bees for interspecific pathogen detection.

Standard Used	Sample Processing	Reference	Pub. Year	Location	No. of Species	Apis	Bombus	Other	Template (μl)	Max Cycle No	No. of Path. Tested	BQCV	DWV	IAPV
No	Pool	⁵⁵	2011	UT, US	1		*		NR	30	1		*
		⁵⁶	2015	MX	1		*		1	40	10		*	*
		⁵⁷	2021	PA, US	3	*	*	*	2	35	5		*	*
Yes		⁵⁸	2016	IA, US	5	*		*	NR	40	5		*	*
		⁵⁹	2018	IT	1			*	5	50	1		*
		¹⁹	2019	VT, US	3	*	*		NR	40	3	*	*	*
No	Indiv	⁶⁰¹	2020	PE; BO	3		*		NR	NR	5
Yes		⁶¹	2015	G.B	2	*	*		NR	40	6	*	*
		⁶²²	2018	UK	5	*		*	2–2.5	45	5	*
		⁶³	2021	IA, US	3	*	*		NR	45	3
	Both	This paper	2022	NC, US	8	*	*	*	1	40	9	*	*	*

This table shows papers that used quantified PCR techniques only and notes if each paper included a standard curve during analysis.
¹This paper used qPCR.
²This paper used RT-PCR for some targets and RT-qPCR for others.

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