Table 1 Sequences of primers used for characterization of LsLGPs.

Gene	Forward (5ʹ → 3ʹ)	Reverse (5ʹ → 3ʹ)	Use
LsLGP1L	AAACAATGGCTATAAAATGAATAATCCCATT	GAATAACCTCGTCATTCAGAATAACACCCT	RACE
LsLGP1	AAAACGAAGAGACAGTTGTCCCAAATT	CGTCATTCAGAATAACATCGTTTTTCTC	RACE
LsLGP1/1L	GGAGTATACAAGTACATCATGAAAGGAACA	CAGAAAGATATTGTGACATTTAGTTCAAAA	ORF
LsLGP1/1L	ATTCTTAATGAAGAATCTGGTGAGGGA	TTTTGAACTAAATGTCACAATATCTTTCTG	IS/RNAi
LsLGP1L	AAACAATGGCTATAAAATGAATAATCCCATT	GAATAACCTCGTCATTCAGAATAACACCCT	qPCR
LsLGP1	AAAACGAAGAGACAGTTGTCCCAAATT	CGTCATTCAGAATAACATCGTTTTTCTC	qPCR
LsLGP2	TGCACCTGCACCAGCACCTGCACCA	TGGTGCTGGTGGCGGTGCAATTCCA	RACE
LsLGP2	TGTTTTAAACTCAATGATGCATTGGCAACC	TTTAATGATCGAAAACGATGGGTAC	ORF
LsLGP2	GCAAATCCGGGAAAAGAAATCCAAG	TCGAAAACGATGGGTACGTCTC	IS/RNAi
LsLGP2	GCTCTCCTTAGTATCTTTATTTTGGGCTCC	ACACGTAAGCACAGCTTGTGGATGC	qPCR
LsLGP3	CCAGTGGGCTCCAATCCTAGTGAA	AGTTTGGGCTGTTGTTCCGGTT	RACE
LsLGP3	ATGAAACTAATATTTTCAAGTTTGATTACA	TTAGATATCTGTGTAATAAGTTTGGGCTGT	ORF
LsLGP3	CCAGTGGGCTCCAATCCTAGTGAA	AGTTTGGGCTGTTGTTCCGGTT	IS/RNAi
LsLGP3	TCAAGAAGGATTTGAAAAGTTGACGAATG	TGCCCGATTGTTCCATAATTCCGTT	qPCR
LsLGP3	CAGTCAGCTCTTCTAGTCAGCACGGTATTCGAGTGAAG		RecLGP4
LsLGP4	GAACAACGCAATCTGGACAA	CGGAGAGTACTTTAACATGAGTCA	RACE
LsLGP4	TGTTTTTTCAAGATCGACTCTTCCAATGG	TTAATCACTCTCGGAGAGTACTTTAACATG	ORF
LsLGP4	GAACAACGCAATCTGGACAA	CGGAGAGTACTTTAACATGAGTCA	IS/RNAi
LsLGP4	TGTTTTTTCAAGATCGACTCTTCCAATGG	AGCACCTTTCTTTTTTGCAAGTTCCATA	qPCR
LsEF1α	GGTCGACAGACGTACTGGTAAATCC	TGCGGCCTTGGTGGTGGTTC	qPCRref (ref)
LsADT3	CTGGAGAGGGAATTTGGCTAACGTG	GACCCTGGACACCGTCAGACTTCA	qPCRref (ref)

Primers were used in rapid amplification of cDNA ends (RACE), confirming the open reading frame (ORF), synthesis of RNA probes for in situ hybridization (IS) and double stranded RNA for RNA interference (RNAi), production of recombinant LsLGP3 (recLGP3), and for real time RT-PCR both target genes (qPCR) and reference genes (qPCRref). Gene name abbreviations: Lepeophtheirus salmonis (Ls), labial gland protein (LGP), elongation factor (EF), and adenine nucleotide translocator (ADT).

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