Table 2 Sequences of primers used for real time RT-PCR analyzing immune responses in Atlantic salmon.
Gene | Forward (5ʹ → 3ʹ) | Reverse (5ʹ → 3ʹ) | Accession no |
|---|---|---|---|
C3a | ATTCTTCCCCTCCACTCCCTCG | CGATTTGGTCGTCAAGCCAGG | XM_014186867 |
IL1β | GCTGGAGAGTGCTGTGGAAGA | TGCTTCCCTCCTGCTCGTAG | XM_014170479 |
IL4/13A | CGTACCGGCAGCATAAAAATCACCATTCC | CCTTGCATTTTGTGGTGGTCCCA | NM_001204895 |
IL6 | ACCAACAGTTTGTGGAGGAGTTTCAGAAGC | CCTGCAGACATGCCTCCTTGTTG | KJ425513 |
IL8 | GCATCAGAATGTCAGCCAGCC | ACGCCTCTCAGACTCATCCC | NM_001140710 |
IL10 | ATGAGGCTAATGACGAGCTGGAGA | GGTGTAGAATGCCTTCGTCCAACA | XM_045705802 |
TNFα | CACTGCCACCAAGAGCCAAG | CGCCAGTTGTCATCGCATACC | DQ787157, DQ787158 |
IFNγ | ATGGATGTGTTATCAAGGGCTGTGATGTG | CAGCTGGTCCTTGGAGATCTTATAGTGGAC | AY795563, XM_045698695 |
MMP13 | ACTCTTTGCCAATATCGCCACCCA | TGGGCCCTCGTTTGAACGCA | BT058668 |
MHCII | GGACGTGAGGTGAAGTCTGATGTGACC | CTGATGTGCTCCACCATGCAGGA | BT058598 |
TCRα | ATGAGCCATCCTACTACACGTTGAACTCAA | CACTCTGGTGGCCTCTGTATTGTTGAAGAC | BT057540 |
CD4 | GAGTACACCTGCGCTGTGGAGT | GGTTGACCTCCTGACCTACAAAGG | EU585750 |
CD8α | TAGAGTGCAAGACAACGCTGGAATGGA | TCTCGAGCCTTTTTGAAAGCCTTCAG | AY693393, AY701521 |
NCCRP1 | AATCCTGCGCCTCACGGTGTGAGTC | GCGAGGAGGTCCTTCTGGTGGAAAC | NM_001166257 |
IgD | CACCAGGAGGAAAGTTTGGCATCA | CCCCAAGGAGCTCTGGTTTGGA | AF141606 |
IgM | TGAGGAGAACTGTGGGCTACACT | TGTTAATGACCACTGAATGTGCAT | BT058539 |
IgT | GGTGGTCATGGACGTACTATTT | CCTGTGCAGGCTCATATCTT | GQ907004 |
EF1α | CACCACCGGCCATCTGATCTACAA | TCAGCAGCCTCCTTCTCGAACTTC | BT043567 |
TRIM16 | TTACTGTAGGAGCTGTATTGAGGGCTGCTG | TTCTCCACCAGCTCAGCCAACATG | XM_014170167 |
