Figure 5
From: Homology directed correction, a new pathway model for point mutation repair catalyzed by CRISPR-Cas
Pooled and single colony NotI digestions of 1228 NotI-MTs. (a) NotI digestions were done on four 5′ and 3′NotI-MT colony PCR pools consisting of 9 single colonies per pool. (b) NotI digestions were done on individual colonies from 3′NotI-MT colony PCR Pool 3 and (c) 5′NotI-MT colony PCR Pool 1. Pools containing uncorrected NotI-MT DNA can be seen in lanes containing intact, undigested linear PCR amplicons as single bands. Pools containing corrected NotI-MT DNA can be seen in lanes with multiple bands after amplicon digestion are shown in red, with the upmost showing uncut, uncorrected PCR amplicon and the lower two showing successful NotI digested fragments. Original gels are presented in Supplementary Fig. 3.
