Figure 6
From: Homology directed correction, a new pathway model for point mutation repair catalyzed by CRISPR-Cas
Sanger sequencing analysis of HDC repair outcomes. Individual clones were sequenced via Sanger sequencing to examine repair profiles of 3′NotI-MT (left) and 5′NotI-MT (right) at the (a) 1364 and (b) 1228 target sites. For each profile, a reference WT sequence of each NotI-MT plasmid is shown in alignment with the HDR correction template and the Cas12a target sites. The point mutations to be corrected are shown with the red boxes and the Cas12a cleavage sites are shown as a vertical dashed line. Aligned under the reference WT sequence, the individual repair profiles can be seen as insertions or modifications within purple boxes and deletions with dashed horizontal lines. Sanger sequencing analysis was done using DECODR (see “Materials and Methods”).
