Figure 7
From: Homology directed correction, a new pathway model for point mutation repair catalyzed by CRISPR-Cas
NGS analysis of HDC repair outcomes. DNA recovered after HDC reactions were sequenced via NGS to examine repair profiles of 3′NotI-MT (top) and 5′NotI-MT (bottom) at the (a) 1228 and (b) 1364 target sites. For each profile, a reference WT sequence of each NotI-MT plasmid is shown in alignment with the individual repair outcomes beneath. The predicted Cas12a cleavage site is shown as a vertical dashed line. The frequency at which each unique indel event was detected is shown to the right as a percentage of the total reads for each NGS reaction. Modifications are shown in bold, insertions within red boxes and deletions with dashed horizontal lines. NGS analysis was done using CRISPResso2 (see “Materials and Methods”).
