Figure 1
MIR181A1HG expression decreases with osteogenic differentiation. (A) Hierarchical clustering of 6185 DE mRNAs in hMSC-hTERT20 cells. (B) Hierarchical clustering of 1153 DE lncRNAs in the same hMSC-hTERT20 (n = 6). (C) MIR181A1HG expression decreases with osteogenic differentiation of tert-immortalized hMSCs (hMSC-hTERT20) (n = 6). ** padj < 10–4; *** padj < 10–7. (D) MIR181A1HG expression across human tissues (n = 1–2). The lncRNA is ubiquitously expressed across all examined cells and tissues except in articular chondrocytes. Datasets from twenty-one adult human tissues were downloaded from the ENCODE project50 and examined for MIR181A1HG expression. (E) MIR181A1HG expression in MSCs derived from white adipose tissue (WAT), muscle (MUS) and bone marrow (BM) undergoing adipogenic and osteogenic differentiation for 9 days28. MIR181A1HG expression is significantly decreased after osteogenic differentiation compared to mulitpotent or adipogenic cells (n = 3). ***padj < 10–7, ****padj < 10–10. Data are presented as mean ± SD.
