Figure 2

Adult cardiomyocytes express elevated protein levels of BRG1/SMARCA4 in hypertrophic cardiomyopathy. (a) BRG1/SMARCA4 protein expression visualized in healthy adult cardiomyocytes (left) and adult cardiomyocytes with HCM (right). Top panel shows H&E stained sample, bottom panel shows high power BRG1/SMARCA4 immunohistochemistry. Scale bars represent 50 µm. (b) HCM tissue in discovery cohort show increased BRG1/SMARCA4 protein expression relative to healthy controls. Data presented as mean + SEM. **** denotes p < 0.0001. (c) HCM tissue in validation cohort show increased BRG1/SMARCA4 protein expression relative to healthy controls. Data show proportions of subjects that fell into each BRG1/SMARCA4 protein expression tier. (d) HCM tissue in validation cohort do not show a significant difference in BRM/SMARCA2 protein expression compared to controls. However, DCM hearts show reduced BRM/SMARCA2 protein expression relative to healthy controls, IHD and SCD hearts. Data presented as mean + SEM. *** denotes p < 0.001, ** denotes p < 0.01. Graphs generated with GraphPad Prism 9.1.1 (GraphPad, San Diego, CA) by JCS.