Figure 2
From: Prediction of gestational age using urinary metabolites in term and preterm pregnancies
Prediction of gestational age at time of collection and associated biological processes. (a) Performance of the restricted RF prediction model of GA that uses three metabolites (C19H26O7S, C24H30O9 and estriol glucuronide) and all the samples in the study (n = 99). The model was validated in an independent cohort (n = 20). The blue area represents the 95% confidence interval. (b) Principal component analysis using predictive metabolites (P-value < 0.05). PC1 and PC4 were chosen because they associated the most strongly with GA. (c) KEGG metabolic pathway enrichment analysis. (d) Volcano plot of annotated significant metabolites (P-value < 0.05). Beta coefficients were calculated using a linear modeling and P-values were calculated from Spearman correlations. (e) Top 6 metabolites in the predictive model and LOESS fit across all the samples. The grey area represents the 95% confidence interval.
