Figure 1

H₂O₂-induced senescence and changes in mitophagy-related proteins in HEI-OC1 cells and cochlear explants. HEI-OC1 cells were exposed to 2 mM H₂O₂ for 1 h and incubated for 3, 4, and 5 days. Whole cochleae also were exposed to 0.5 mM H₂O₂ for 5 h and incubated for 1, 3, and 5 days. The levels of p53 and p21 (cellular senescence markers) increased in H₂O₂-treated HEI-OC1 cells (A) and cochlear explants (B). The levels of mitophagy-related proteins (PINK1, Parkin, BNIP3, and LC3B) significantly decreased in H₂O₂-treated HEI-OC1 cells (A) and cochlear explants (B). HEI-OC1 cells were exposed to 2 mM H₂O₂ for 1 h and incubated for 5 days. β-galactosidase staining of senescent cells significantly increased in H₂O₂-treated cells (C, D). The population doubling level significantly decreased in H₂O₂-treated cells (E). Data are presented as means ± standard errors of the mean of five independent experimental results (SA-β-gal, senescence associated-beta galactosidase). *p < 0.05, **p < 0.01. The grouping of gels/blots cropped from different parts of the same gel. Full-length blots are presented in Fig. S1A, S1B.