Table 2 Metabarcoding and metatranscriptome profile of Pinus contorta needle samples.

From: Transitions of foliar mycobiota community and transcriptome in response to pathogenic conifer needle interactions

Disease symptom

Sample

DNA contigs (metabarcoding)

Raw RNA reads*

Paired reads that aligned concordantly to the assembly ≥ 1 time (%)

Overall rate (%) of alignment to the assembly

L. concolor

L. montivaga

Asymptomatic

CS02-18CN

1014

173

21,337,962

78.82

93.4

TC01-19CN

2386

3706

24,537,902

79.75

93.56

Symptomatic

CS02-18CP

26,149

84

20,416,050

66.61

87.89

SR10-19CP

22,791

0

21,485,100

71.65

91.68

Asymptomatic

LV02-18MN

22,078

18,744

22,184,468

78.33

89.96

LV03-18MN

522

4785

25,400,132

73.79

86.48

SR09-18MN

26,245

1422

30,543,904

77.44

94.48

Symptomatic

LV02-18MP

0

20,353

20,609,968

79.88

92.06

NC04-18MP

3

63,789

24,456,808

77.26

92.70

SR09-18MP

0

43,121

26,930,926

78.37

92.76

  1. Proportions (%) of raw reads that aligned to the metatranscriptome assembly and the overall alignment rate are also shown.
  2. Cross () represents samples excluded from the analyses.
  3. *Number of RNA reads per sample annotated as fungi through concatenated databases of NCBI-nr and JGI Mycocosm is listed on Table S4.