Figure 3
From: Doxorubicin impacts chromatin binding of HMGB1, Histone H1 and retinoic acid receptor
Dox decreases the binding of rHMGB1 to plasmid DNA. (a) Diffusion coefficient of SYBR Gold stained superhelical plasmid DNA (4700 bp) and of Alexa-647 labelled rHMGB1. The protein had a single diffusing species when alone in the solution and two in the presence of the plasmid DNA as measured by FCS. (b) Fraction of rHMGB1 bound to supercoiled plasmid DNA in the presence of varying concentrations of Dox. One-way ANOVA with post hoc Dunnett’s test was used to calculate significance ****p < 0.0001.
