Figure 1

Effects of lenvatinib on the MAPK signaling pathway in HCC cells. (A,B) Lenvatinib was added at concentrations of 0, 1, and 3 μM in medium containing 10% fetal bovine serum for 1, 4, and 24 h, respectively. Lenvatinib downregulated ERK phosphorylation in Hep3B cells (A) and Huh7 cells (B). (C) Lenvatinib at 3 µM for 4 h downregulated ERK phosphorylation in JHH-4 and Huh6 cells. (D) Lenvatinib at 3 µM for 4 h did not downregulate ERK phosphorylation in HepG2 cells. In all experiments, immunoblotting of phosphorylated ERK (p-ERK), ERK, and internal controls (ACTB or TUBB) levels was performed. The intensity with the internal control were indicated. At least two times of the independent experiments of (A-D) were performed and representative results were shown.