Figure 3
From: Human induced mesenchymal stem cells display increased sensitivity to matrix stiffness
Mechanosensitivity of iMSC lines. Single-cell image analysis of (a) nuclear YAP localization, (b) pFAK length, (c) number of adhesions per cell, and (d) actin fiber anisotropy of iMSCs cultured atop Low, Med, and High stiffness hydrogels. (e) Representative quantifications of high and low nuclear YAP ratios (YAP, green; nucleus, white dashed oval). Scale bar, 50 µm. (f) Representative image and quantification of iMSC cultured atop Med stiffness hydrogel (red, actin; blue, nucleus). Scale bar, 50 µm. Representative image and quantification of iMSC cultured atop a (g) Low and (h) High stiffness hydrogel (green, pFAK; red, actin; blue, nucleus). Scale bar, 50 µm. Bar graphs represent the mean and error bars represent standard deviation. Box plots show 25/50/75th percentiles, whiskers show minimum/maximum; n > 50 cells per group, ***p < 0.001.
