Figure 4
From: Human induced mesenchymal stem cells display increased sensitivity to matrix stiffness
Morphological and cellular mechanosensing comparison between iMSCs and MSCs on Med stiffness hydrogels. (a) Representative cell silhouettes of 3 iMSC (top) and MSC (bottom) cell lines. Histograms of (b) cell area and (c) nuclear YAP of 3 iMSC (top) and MSC (bottom) cell lines. (d) Nuclear YAP versus area scatter plot of 3 iMSC (top) and MSC (bottom) cell lines. Whisker plots of (e) pFAK length and (f) actin anisotropy of iMSC (3 left whisker plots) and MSC (right whisker plots) cell lines. (g) Scatter plots of pFAK length versus actin anisotropy of 3 iMSC (top) and MSC (bottom) cell lines. Scale bars: 50 µm. Box plots show 25/50/75th percentiles, whiskers show minimum/maximum; n > 50 cells per group, *p < 0.05, **p < 0.01, ***p < 0.001, n.s. not significant.
