Figure 4

T cells localize to the epineurium of the sciatic nerve, where their cytokines can influence the neuronal cells and Schwann cells. (a/e) Demonstrate the ability to differentiate the fasciculated nerve from the epineurium using differential interference contrast (DIC). (b/f) The CD3e pan-T cell marker shows localization of T cells in the epineurium. (c/g) Shows that the CD4 subtype marker (red) co-localizes with CD3e (green) heavily in the epineurium with sparse occasional CD4 only staining occurring within the fasciculated nerve. (d/h) Shows that CD8 (red) occasionally co-localizes with CD3e in the epineurium but also is more frequently present without CD3e within the fasciculated nerve. Panels d and h are located from a different slide than panels (a–c) and (e–g) due to compatibility of primary antibodies.