Figure 1

Shotgun label-free quantitative peptidomic analysis. (A) Overview of the protocols applied for the analysis of peptides in NAS-positive and healthy milk samples (B) Venn diagram of all the peptides identified in milk samples from healthy and NAS-positive buffaloes. Peptides were considered differentially abundant if they were present only in NAS-positiver or Control milk or showed significant Welch t test difference (cut-off at 1% permutation-based False Discovery Rate).