Figure 10 | Scientific Reports

Figure 10

From: Sulforaphane suppresses the activity of sterol regulatory element-binding proteins (SREBPs) by promoting SREBP precursor degradation

Figure 10

SFaN promotes the Keap1-Nrf2 pathway-independent SREBP precursor degradation. (A,B) Huh-7 cells were transfected with 24 pmol of siRNA (siCon) or Nrf2 siRNA and incubated in medium B for 48 h. The cells were then shifted into medium B in the presence of the vehicle or the indicated concentrations of SFaN for 3 h. Whole-cell extracts underwent immunoblotting (IB) with anti-SREBP-1 (2A4), anti-SREBP-2, or anti-β-actin antibodies (A). The asterisk denotes nonspecific bands. Full-length images are presented in Supplementary Fig. S10. Total RNA was isolated from the cells. Real-time quantitative PCR was performed, and relative mRNA levels were obtained by normalization to GAPDH mRNA. All data are represented as the mean ± S.E. (n = 3) (B). Different superscript letters denote statistical significance (p < 0.05).

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