Figure 4 | Scientific Reports

Figure 4

From: Sulforaphane suppresses the activity of sterol regulatory element-binding proteins (SREBPs) by promoting SREBP precursor degradation

Figure 4

SFaN accelerates SREBP precursor degradation. (A,B) Huh-7 cells were sterol-supplemented by incubating in medium E for 16 h to increase the SREBP precursor forms. After incubation with 50 μM cycloheximide for 30 min, the cells were shifted into medium E supplemented with 50 μM cycloheximide in the presence of the vehicle, 100 μM SFaN (A), or SFeN (B). After incubation for the indicated periods, whole-cell extracts underwent immunoblotting (IB) with anti-SREBP-1 (2A4), anti-SREBP-2, or anti-β-actin antibodies. The signals were quantified by Image J and normalized by β-actin signals. Full-length images are presented in Supplementary Fig. S4.

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