Figure 3

4930567H17Rik^∆CDS/Y and Mageb5^{∆Arm∆CDS/Y} mice display testis histology, testis size, sperm counts, and motility similar to 4930567H17Rik^+/Y and Mageb5^+/Y mice. (A) Periodic Acid-Schiff and Hemoxylin stained sections showing normal progression of spermatogenesis in 4930567H17Rik^∆CDS/Y and Mageb5^{∆Arm∆CDS/Y} mice. Arrows indicate presence of round spermatids. (B) Total testis weight normalized to total body weight (g). 4930567H17Rik^+/Y n = 8, 4930567H17Rik^∆CDS/Y n = 11, Mageb5^+/Y and Mageb5^{∆Arm∆CDS/Y} n = 9 (C) Sperm counts. Each point represents a single individual where 2 technical replicates were counted and averaged. 4930567H17Rik^+/Y n = 7, 4930567H17Rik^∆CDS/Y n = 8, Mageb5^+/Y and Mageb5^{∆Arm∆CDS/Y} n = 9. (D) Percent motile sperm. 4930567H17Rik^+/Y and 4930567H17Rik^∆CDS/Y n = 5, Mageb5^+/Y n = 7 and Mageb5^{∆Arm∆CDS/Y} n = 8. (E) Pups/litter from mating three males from each genotype with CD1 females. 4930567H17Rik^+/Y n = 18, 4930567H17Rik^∆CDS/Y and Mageb5^+/Y n = 17 and Mageb5^{∆Arm∆CDS/Y} n = 18 litters. (F) Sex genotyping performed on pups from litters shown in (E). Number of male offspring are shown as percentage of total number of pups in parenthesis below. All comparisons in (B–F) were performed using an unpaired two-tailed student’s t-test between 4930567H17Rik^+/Y versus 4930567H17Rik^∆CDS/Y and Mageb5^+/Y versus Mageb5^{∆Arm∆CDS/Y}. All error bars represent mean with standard deviation.