Table 2 Comparison between RNA sequencing findings and qPCR.

From: Short and long-term effect of dexamethasone on the transcriptome profile of primary human trabecular meshwork cells in vitro

S. no.

Name of the gene

Log2 fold change

Group A

Group B

RNA seq

qPCR

RNA seq

qPCR

1

FKBP5

2.91

2.97

4.47

3.94

2

FST

 − 2.45

 − 2.61

 − 2.04

 − 2.88

3

MMP1

 − 1.97

0.30

 − 1.47

 − 4.53

4

PDPN

1.35

1.24

 − 0.001

 − 0.60

5

PTK2B

2.34

1.79

1.8

0.38

6

RGCC

4.79

3.54

3.59

4.48

7

SERPINB2

 − 3.73

 − 2.10

 − 0.41

1.32

8

VEGFC

 − 1.32

 − 1.30

 − 0.82

 − 0.10

9

ZBTB16

7.70

3.19

6.47

8.90

  1. Group A: DEGs between DEX and ETH treated 16 h; Group B: DEGs between DEX and ETH treated for 7 d.
  2. FKBP5 FK506 binding protein 5, FST follistatin, MMP1 matrix metalloproteinase-1, PDPN podoplanin; protein tyrosine kinase 2 beta, RGCC regulator of cell cycle, SERPINB2 serpin family B member 2, VEGFC vascular endothelial growth factor-C, ZBTB16 zinc finger and BTB domain containing 16.
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