Figure 3

DPD expression was associated with response to gemcitabine-containing chemotherapy. (a) (upper panel) Protein levels of DPD in TUR sections from patients with a good response and patients with a poor response excluding the sample from #06 due to poor quality were detected by western blotting. (lower panel) Quantitative protein levels of DPD in good and poor responders were displayed. *P < 0.05. (b) Receiver operating characteristic curves for prediction of response to chemotherapy by protein level of DPD are shown. AUC, area under curve. (c) Immunohistochemistry of TUR sections from patients with a good response and patients with a poor response are shown. Brown stain represents DPD protein expression. Magnification, × 400. (d) DPYD mRNA expression levels in 223 patients (GSE87304) were shown by molecular subtypes. *P < 0.05, **P < 0.01, ***P < 0.001. (e) Pathological T-stage in patients (GSE87304) with highest (n = 42) and lowest (n = 42) quartile of DPYD expression when treated with gemcitabine-containing chemotherapy. *P < 0.05. (f) Overall survival in patients (GSE87304) between in patients with highest (n = 42) and lowest (n = 42) quartile of DPYD expression when treated with gemcitabine-containing chemotherapy. *P < 0.05. Vertical bar on Kaplan–Meier curve represents censor.