Table 1 Summary table of Nanodrop DNA and mRNA concentration, TTP and standard deviation (sd) or cycle threshold (Ct) for pH-LAMP or PCR test on LC96 system and LoC platform. *HPV-18 DNA was detected.
Sample name | Diagnosis | DNA conc. (ng/\(\upmu \)L) | RNA conc. (ng/\(\upmu \)L) | GAPDH RNA TTP (sd) | HPV-16/18* DNA TTP (sd) | HPV-16/18* LOC TTP | HPV-16/18* PCR Ct (sd) | hTERT RNA TTP (sd) | hTERT LOC TTP | hTERT PCR CT (sd) |
|---|---|---|---|---|---|---|---|---|---|---|
H2 | Benign | 322.30 | 22.80 | 9.02 (0.11) | – | 17.65 | 35.51 | – | – | – |
H5 | SCC | 1745.00 | 1434.40 | 7.06 (0.11) | 20.56 (0.56) | 15.57 | 21.41 (0.02) | 12.42 (0.34) | 21.38 | 33.48 (0.03) |
H7 | SCC | 1811.80 | 1576.50 | 5.70 (0.05) | 18.87 (0.23) | 13.65 | 35.95 (0.40) | 12.83 (0.77) | 21.72 | 33.70 (0.69) |
H9 | Benign | 590.30 | 195.40 | 6.96 (0.04) | – | – | – | – | – | – |
H11 | Benign | 169.50 | 44.40 | 7.96 (0.16) | 12.48* (0.49) | 7.42* | 27.16* (0.11) | – | – | – |
H12 | SCC | 1412.70 | 718.60 | 6.69 (0.13) | – | 17.48 | 36.30 (0.12) | 14.21 (2.79) | 18.67 | 31.57 (0.09) |
H17 | Benign | 2192.80 | 957.40 | 8.67 (0.12) | 35.40 (5.08) | 15.40 | 34.81 (1.93) | – | – | – |
H18 | Benign | 22.70 | 9.20 | 9.20 (0.09) | – | – | – | – | – | – |
H27 | ADC | 1510.50 | 1570.90 | 7.97 (0.05) | 20.74 (0.26) | 18.43 | 32.61 (0.31) | 15.06 (4.01) | 14.77 | 32.75 (0.30) |
H45 | SCC | 1439.30 | 1048.20 | 6.47 (0.07) | 18.63* (0.21) | 18.60* | 29.73* (0.02) | 14.82 (2.22) | 8.88 | 32.06 (0.33) |
