Figure 1

Correlative analyses of phylogenetic relationship, isolation sources, and frequency of mutations in efhP homologs. (A) The 1723 sequences of efhP homologs and efhP were clustered using CD-Suite²⁶ with a cutoff at 0.9995. One representative from each cluster was used to build a phylogenetic tree in MEGA²⁷. The branch lengths show the evolutionary time between two nodes. PA1249, which encodes an EF-hand containing protease, AprA, was included as an outgroup. (B) Clustering sequence similarities with the isolation sources formed two apparent groups divided by the horizontal line. Top group represents 1567 sequences, and the bottom group represents 156 sequences. The isolation sources shown are grouped into main categories that represent at least 60% of the sequences shown in (Fig. S2C). All other sources are represented as ‘Others’. (C) Mutation profiles are depicted for each group. The % of efhP homologs carrying the depicted mutations are plotted. The arrows indicate missense mutations.